THEME04-9-1077-2015/2017

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04-9-1077-2015/2017

Priority:

Status:

In-progress

Research on the Biological Effect of Heavy Charged Particles
with Different Energies

Leaders:

E.A. Krasavin
G.N. Timoshenko

Participating Countries, Institutes and International organizations:
Armenia, Belarus, Bulgaria, Czech Republic, Egypt, Italy, Moldova, Mongolia, Poland, Romania, Russia, Slovak Republic.

Scientific Programme:
Theoretical and experimental research on the biological effect of heavy charged particles with different energies at JINR's basic facilities.
The research and development will include:

Research on the regularities and mechanisms of the initiation of molecular disorders in the DNA structure and their repair; and radiation-induced apoptosis in human cells under exposure to radiations with different linear energy transfer (LET).
Decoding of the mechanisms underlying cell hypersensitivity and hyperresistance to low doses of ionizing radiation.
Acquisition of comparative data on the regularities of the induction of gene and structural mutations in mammalian and lower eukaryote cells by sparsely and densely ionizing radiations with different LET.
Research on the mechanisms of the induction of retinal damage by heavy charged particles (HCP) and its repair.
Research on the character of the HCP-induced damage of central nervous system (CNS) cells and regularities of their death. Identification of HCP-induced functional and morphological disorders in the CNS.
Mathematical modeling of the effects induced by ionizing radiations with different LET at the molecular and cellular levels. Development and analysis of mathematical models of the molecular mechanisms of CNS structural and functional disorders induced by ionizing radiations.
Calculation of the radiation shielding of new nuclear physics facilities; evaluation of the radiation envi\-ron\-ment and development of radiation safety systems.

Expected main results in 2016:

To continue studying regularities in the induction, formation, and repair kinetics of HCP-induced clustered DNA double-strand breaks (DSBs) in human skin fibroblast nuclei.
To study the expression of the genes encoding the proteins RAD51, DNA PKcs, NBS1, and MRE11, which participate in HCP-induced damage repair in human fibroblasts.
To continue studying radiation-induced apoptosis mechanisms and different ways of HCP-induced DNA damage repair. To study the expression of the genes that encode the proteins and caspases participating in apoptosis induction in human fibroblasts by HCP.
Using a Synergy H1m microplate reader, to evaluate the level of the reactive oxygen species and ERK protein kinase in mammalian cells exposed to gamma-rays and HCP.
To continue studying regularities in the induction of gene and structural mutations in yeast cells by radiations with different LET.
To continue research on the mutagenic effect of ionizing radiations on mammalian cells in the remote after irradiation. To perofrm a molecular analysis of HPRT-mutant subclones.
To continue evaluating the retina's ability to recover after radiation exposure. To continue studying the mechanisms behind retina recovery: Mueller cell activation, expression of endogenous protectors in the retina, and expression of the oxidative stress proteins.
To continue research on the apoptotic death of neurons in different sections of the rat and mouse brain at different times after exposure to radiations with different LET.
To continue studying disorders in the exchange of monoamines and their metabolites in the prefrontal cortex, hippocampus, striatum, adjoining nucleus, and hypothalamus of the rat brain under exposure to gamma-rays, Bragg peak protons, and HCP.
To continue the development of mathematical models of neurons' functional activity under exposure to radiations with different LET.
To continue elaboration of model approaches to the quantitative estimation of the relation between the plastic processes in neurons participating in the formation of the brain's functional networks.
To work out nonlinear dynamics methods for a neuron population (neural networks) that would allow describing the classical and quantum mechanisms of cognitive function realization.
To develop mathematical models of axon transport of signals and molecules.
To perform mathematical modeling of functional changes in the mammalian retina under exposure to genotoxic factors of different nature.
To continue the development of mathematical models of DNA double-strand break induction and repair in mammalian and human cells.
To ensure the conduction of the radiobiological experiments at the Nuclotron (the Laboratory of High-Energy Physics), U-400M cyclotron (the Laboratory of Nuclear Reactions), and the medical beam of the Phasotron (the Laboratory of Nuclear Problems).

List of projects:
	Project	Leader	Priority (period of realisation)
1.	Research on the biological effect of heavy charged particles with different energies	E.A. Krasavin G.N. Timoshenko	1 (2015-2017)

List of Activities

Activity or Experiment

Leader

Status

Laboratory or other
Division of JINR
Responsible person

Main researchers

Radiobiological research
at charged particle beams

E.A. Krasavin

Data taking
Realization
Data taking

LRB

S.V. Aksenova, G.F. Aru, K.V. Belokopytova,
O.V. Belov, P. Blaha, Yu.V. Bogdanova, A.V. Boreyko,
A.N. Bugay, N.N. Budennaya, T.S. Bulanova,
V.N. Chausov, E.B. Dushanov,D.V. Elsha, T.A. Fadeeva,
T.B. Feldman, R.D. Govorun, E.V. Ilyina, A.A. Ivanov,
L. Jezkova, A.N. Kokoreva, E.A. Kolesnikova,
N.A. Koltovaya, O.V. Komova, I.V. Koshlan,
N.A. Koshlan, M.A. Kovalenko, A. Kowalska, P.V. Kutsalo,
E.A. Kuzmina, V.N. Lisy, K.N. Lyakhova, M.S. Lyashko,
B. Munkhbaatar, K.O. Muranov, E.A. Nasonova,
S.S. Negovelov, M.A. Ostrovsky, A.Yu. Parkhomenko,
I.I. Ravnachka, Yu.S. Severyukhin, N.L. Shmakova,
E.V. Smirnova, S.I. Tiunchik, V.A. Tronov, D.M. Utina,
M.A. Vasilyeva, Yu.V. Vinogradova S.V. Vorozhtsova,
M.G. Zadnepryanets, E.M. Zaytseva, N.I. Zhuchkina,
+ 2 engineers, + 7 workers

Radiation research

G.N. Timoshenko

Preparation
Data taking
R&D

LRB

V.E. Aleinikov, L.G. Beskrovnaya, A.R. Krylov, V.A. Krylov,
E.N. Lesovaya, + 10 engineers, + 2 workers

Training activity

E.A. Krasavin
S.Z. Pakuliak (UC)

LRB

V.E. Aleinikov, O.A. Bakerin, O.V. Belov, A.V. Boreyko,
N.N. Budennaya, T.B. Feldman, R.D. Govorun,
A.A. Ivanov, N.A. Koltovaya, O.V. Komova,
M.M. Komochkov, I.V. Koshlan, Yu.V. Mokrov,
M.A. Ostrovsky, A.Yu. Parkhomenko, G.N. Timoshenko

Collaboration
Country or International Organization	City	Institute or Laboratory
Armenia	Yerevan	YSU
Belarus	Gomel	IRB NASB
Bulgaria	Sofia	IE BAS
		INRNE BAS
		NCRRP
Czech Republic	Brno	IBP ASCR
	Prague	CTU
	Rez	NPI ASCR
		UJV
Egypt	Giza	CU
Italy	Udine	Uniud
Moldova	Chisinau	UnASM
Mongolia	Ulaanbaatar	NUM
Poland	Szczecin	US
Romania	Bucharest	UMF
		ISS
	Iasi	UAIC
		IBR
Russia	Moscow	IBMP RAS
		IHNA Ph RAS
		SF IPh RAMS
		ITEP
		MSU
	Astrakhan	ASU
	Sochi	SRI MP
	Vladivostok	FEFU
Slovakia	Bratislava	CU

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Research on the Biological Effect of Heavy Charged Particles with Different Energies

Research on the Biological Effect of Heavy Charged Particles
with Different Energies