THEME04-9-1077-2015/2017

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04-9-1077-2015/2017

Priority:

Status:

Extended

Research on the Biological Effect of Heavy Charged Particles
with Different Energies

Leaders:

E.A. Krasavin
G.N. Timoshenko

Participating Countries, Institutes and International organizations:
Armenia, Bulgaria, Czech Republic, Egypt, Italy, Moldova, Mongolia, Poland, Romania, Russia, Slovakia.

Scientific Programme:
Theoretical and experimental research on the biological effect of heavy charged particles with different energies at JINR's basic facilities.

Research on the regularities and mechanisms of the initiation of molecular disorders in the DNA structure and their repair; and radiation-induced apoptosis in human cells under exposure to radiations with different linear energy transfer (LET).
Decoding of the mechanisms underlying cell hypersensitivity and hyperresistance to low doses of ionizing radiation.
Acquisition of comparative data on the regularities of the induction of gene and structural mutations in mammalian and lower eukaryote cells by sparsely and densely ionizing radiations with different LET.
Research on the mechanisms of the induction of retinal damage by heavy charged particles (HCP) and its repair.
Research on the character of the HCP-induced damage of central nervous system (CNS) cells and regularities of their death. Identification of HCP-induced functional and morphological disorders in the CNS.
Mathematical modeling of the effects induced by ionizing radiations with different LET at the molecular and cellular levels. Development and analysis of mathematical models of the molecular mechanisms of CNS structural and functional disorders induced by ionizing radiations.
Calculation of the radiation shielding of new nuclear physics facilities; evaluation of the radiation environment and development of radiation safety systems.

Expected main results in 2015:

To continue studying regularities in the induction, formation, and repair kinetics of HCP-induced clustered DNA double-strand breaks (DSBs) in human skin fibroblast nuclei.
To study the expression of the genes that encode the proteins participating in HCP-induced damage repair in human fibroblasts.
To continue studying radiation-induced apoptosis mechanisms and different ways of HCP-induced DNA damage repair. To study the expression of the genes that encode the proteins and caspases participating in apoptosis induction in human fibroblasts by HCP.
To continue studying regularities in the formation and repair of DNA DSBs and clustered damage using DNA foci. To study DNA DSB repair kinetics by the premature chromatin condensation method.
To continue studying regularities in the induction of gene and structural mutations in yeast cells by radiations with different LET.
To continue research on the mutagenic effect of sparsely and densely ionizing radiations on mammalian cells in the remote period after irradiation. To perofrm an analysis of the chromosome and genome instability of mutant subclones.
To carry out experiments on retinal response to HCP exposure and combined irradiation with -rays and protons. To evaluate the retina's ability to recover after radiation exposure. To determine the mechanisms behind retina recovery: Mueller cell activation, expression of endogenous protectors in the retina, and expression of the oxidative stress proteins.
To estimate the level of the apoptotic death of neurons (based on caspase-3 activity) in different sections of the rat and mouse brain at different times after exposure to radiations with different LET.
To study disorders in the exchange of monoamines and their metabolites in the prefrontal cortex, hippocampus, striatum, adjoining nucleus, and hypothalamus of the rat brain under exposure to -rays, Bragg peak protons, and HCP.
In experiments on primates, to study HCP exposure effects on cognitive functions, electroencephalograms, and the content of monoamines and their metabolites in peripheral blood.
To continue the development of mathematical models of neurons' functional activity under exposure to radiations with different LET.
To elaborate model approaches to the quantitative estimation of the relation between the plastic processes in neurons participating in the formation of the brain's functional networks.
To work out methods of building volume models of nerve cells for microdosimetric calculations.
To develop mathematical models of signal propagation in microtubes and action potential propagation in neurons; and a mathematical model of axon transport of signals and molecules.
To perform mathematical modeling of functional changes in the mammalian retina under exposure to genotoxic factors of different nature.
To continue the development of mathematical models of DNA DSB induction and repair in mammalian and human cells.
To ensure the conduction of the radiobiological experiments at the Nuclotron (the Laboratory of High-Energy Physics), U-400M cyclotron (the Laboratory of Nuclear Reactions), and the medical beam of the Phasotron (the Laboratory of Nuclear Problems).

List of projects:
	Project	Leader	Priority (period of realisation)
1.	Research on the biological effect of heavy charged particles with different energies	E.A. Krasavin G.N. Timoshenko	1 (2015-2017)

List of activities

Activity or experiment

Leaders

Status

Laboratory or other
Division of JINR

Main researchers

Radiobiological research
at charged particle beams

E.A. Krasavin

Data taking
Realization
Data taking

LRB

S.V. Aksenova, G.F. Aru, K.V. Belokopytova, O.V. Belov,
P. Blaha, A.V. Boreyko, A.N. Bugay, N.N. Budennaya,
T.S. Bulanova, V.N. Chausov, E.B. Dushanov,D.V. Elsha,
T.A. Fadeeva, R.D. Govorun, E.V. Ilyina, A.A. Ivanov,
L. Jezkova, A.N. Kokoreva, E.A. Kolesnikova,
N.A. Koltovaya, O.V. Komova, I.V. Koshlan, N.A. Koshlan,
M.A. Kovalenko, A. Kowalska, E.A. Kuzmina,
K.N. Lyakhova, M.S. Lyashko, B. Munkhbaatar,
E.A. Nasonova, S.S. Negovelov, A.Yu. Parkhomenko,
I.I. Ravnachka, A.V. Rzyanina, N.L. Shmakova,
E.V. Smirnova, S.I. Tiunchik, M.A. Vasilyeva,
S.V. Vorozhtsova, A.Kh. Yagova, M.G. Zadnepryanets,
E.M. Zaytseva, N.I. Zhuchkina + 2 engineers, + 6 workers

Radiation research

G.N. Timoshenko

Preparation
Data taking
R&D

LRB

V.E. Aleinikov, L.G. Beskrovnaya, A.R. Krylov, V.A. Krylov,
E.N. Lesovaya + 10 engineers, + 2 workers

Photoradiobiological research

M.A. Ostrovsky

	LRB	T.B. Feldman, P.V. Kutsalo, K.O. Muranov, Yu.S. Severyukhin, V.A. Tronov, D.M. Utina, Yu.V. Vinogradova + 1 worker
4.	Training activity	E.A. Krasavin S.Z. Pakuliak

	LRB	V.E. Aleinikov, O.A. Bakerin, O.V. Belov, A.V. Boreyko, N.N. Budennaya, T.B. Feldman, R.D. Govorun, A.A. Ivanov, N.A. Koltovaya, O.V. Komova, M.M. Komochkov, I.V. Koshlan, Yu.V. Mokrov, M.A. Ostrovsky, A.Yu. Parkhomenko, G.N. Timoshenko
	UC	S.Z. Pakuliak

Collaboration
Country or International Organization	City	Institute or Laboratory
Armenia	Yerevan	YSU
		IMB NAS RA
Bulgaria	Sofia	IE BAS
		INRNE BAS
		NCRRP
Czech Republic	Brno	IBP ASCR
	Prague	CTU
	Rez	NPI ASCR
		NRI
Egypt	Giza	CU
Italy	Udine	UNIUD
Moldova	Chisinau	UnASM
Mongolia	Ulaanbaatar	NUM
Poland	Szczecin	US
Romania	Bucharest	UMF
		ISS
	Iasi	UAIC
		IBR
Russia	Astrakhan	ASU
	Moscow	IBMP RAS
		IHNA Ph RAS
		SF IPh RAMS
		ITEP
		MSU
	Vladivostok	FEFU
Slovakia	Bratislava	CU

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Research on the Biological Effect of Heavy Charged Particles with Different Energies

Research on the Biological Effect of Heavy Charged Particles
with Different Energies