THEME04-2-1132-2017/2023

Biomedical and clinical research of proton radiotherapy for cancer treatment. Formation of the experimental data base in the field of radiation mutagenesis in animal germ cells.

Expected results in the current year:

Evaluation of effectiveness of conducted radiation treatment of different neoplasms.
Increase of functional capability of the developed 3D treatment planning software.
Design and construction of prototype equipment for dynamic conformal irradiation of deep-seated tumours with the proton beam.
Development and improvement of detectors and tools for clinical dosimetry of medical hadron beams.
Continuation of research to determine forms of fibroblast cell death depending on the dose of ionizing radiation.
Study of mechanisms of functional and neurochemical disorders in the central nervous system during exposure to radiation with different linear energy transfer.
Mastering of new methods for evaluation of effectiveness of cytotoxic activity of nanoparticles on tumour cells.
Studies of difference in radiobiological reactions for normal and cancer cells irradiated by flash technique.
Continuation of the molecular analysis of gamma- and neutron-induced intragenic structural changes in germline cells.
Continuation of the sequence analysis of inherited DAN changes at the genome level of the offspring.
Continuation of the work on the analysis of the transcriptome in somatic cells differing in radiosensitivity.
Determination of the secondary structure of the Dsup protein using SAXS, DLS, and circular dichroism methods.
Transcriptome analysis of D.melanogaster strains and human cell line HEK293 expressing Dsup protein.
Assessment of metabolic activity (MTT-test) and apoptosis induction (according to caspase-3/7 activity) in human HEK293 culture cells expressing Dsup, determination of the level of reactive oxygen species in cells.
Development of a project of a specialized isochronous cyclotron for proton therapy.
Measurements of the magnetic field of the MC1 bending magnet for the transport line of the AIC-144, Krakow, Poland.

List of projects
	Project	Leader	Priority (period of realisation)
1.	Further development of methods, technologies, schedule modes and provision of radiotherapy	G.V. Mitsyn	1 (2017-2023)
2.	RADIOGENE: Molecular genetics of radiation-induced changes at the gene, genome and transcriptome level in Drosophila melanogaster	K.P. Afanasyeva Deputy: A.E. Rusakovich	1 (2017-2023)

Study of the radioprotective
properties of the Damage supressor (Dsup)
protein on a model organism D.melanogaster
and the human cell culture HEK293

E.V. Kravchenko

1 (2021-2023)

List of Activities

Activity or Experiment

Leaders

Status

Laboratory or other
Division of JINR

Main researchers

Further development of methods,
technologies, schedule modes
and provision of radiotherapy

G.V. Mitsyn

R&D

DLNP

I.V. Alexandrova, G.A. Andreev, A.V. Agapov, O.V. Belov,
K. Belokopytova, V.M. Breev, G.V. Donskaya, V.N. Gaevsky,
Ye.A. Gritskova, I.I. Klochkov, I. Khosenova, Ye.I. Luchin,
I.Ye. Miller, A.G. Molokanov, S.A. Pisareva, A.V. Rzyanina,
K.N. Shipulin, S.V. Shvidky

RADIOGENE: Molecular genetics
of radiation-induced changes at the gene, genome and transcriptome level
in Drosophila melanogaster

I.D. Alexandrov

R&D

DLNP

M.V. Alexandrova, K.P. Afanasyeva, N.E. Kharchenko,
S.V. Korablinova, L.N. Korovina, N.V. Orlova,
A.N. Rusakovich, O.P. Solodilova

Study of the radioprotective properties of the Damage supressor (Dsup) protein on a model organism D.melanogaster and the human cell culture HEK293

E.V. Kravchenko

R&D

DLNP

T.O. Azorskaya, O.A. Kuldoshina, A.V. Rzyanina,
R.A. Tarasov, A.S. Yakhnenko, M.P. Zarubin

FLNR

T.N. Murugova

Development of methods and programs
for creating cyclotron-type accelerators. Development and upgrade of cyclotrons
for medical application

G.A. Karamysheva

Realization

DLNP

K.S. Bunyatov, A.F. Chesnov, S.N. Dolya, S.B. Fedorenko,
R.V. Galkin, A.L. Gibinsky, S.V. Gursky, G.G. Kazakova,
O.V. Karamyshev, I.N. Kiyan, O.E. Lepkina,
O.V. Lomakina, I.V. Lyapin, V.A. Malinin, D.S. Petrov, D.V. Popov, G.D. Shirkov, S.G. Shirkov, V.L. Smirnov, A.S. Vorozhtsov, S.B. Vorozhtsov

MLIT

I.V. Amirhanov, T.V. Karamysheva

Collaboration

Country or International Organization	City	Institute or Laboratory
Belgium	Louvain-la-Neuve	IBA
China	Hefei	ASIPP
Czech Republic	Prague	ADVACAM
		PTC
	Rez	UJV
Moldova	Chisinau	MSU
Poland	Krakow	INP PAS
	Otwock (Swierk)	NCBJ
	Poznan	GPCC
Romania	Bucharest	IFIN-HH
	Cluj-Napoca	UBB
	Iasi	UAIC
Russia	Moscow	FMBC
		IBMP RAS
		VIGG RAS
	Puschino	ITEB RAS
	Rostov-on-Don	SFedU
	Saratov	SSU
South Africa	Somerset West	iThemba LABS
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Biomedical and Radiation-Genetic Studies Using Different Typesof Ionizing Radiation

Biomedical and Radiation-Genetic Studies Using Different Types
of Ionizing Radiation