Modern
Trends and Developments in Raman Microspectroscopy | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Theme leaders: |
G.M. Arzumanyan |
Participating
countries and international organizations:
Armenia, Belarus, India, Kazakhstan, Poland, Romania, Russia, Serbia, Slovakia, Uzbekistan.
Issues
addressed and main goals of research:
Fundamental and applied research in the field of spontaneous and nonlinear Raman microspectroscopy aimed at highly sensitive biosensing. Study of mechanisms and nature of surface-enhanced Raman scattering (SERS) taking into account the anomalous ratio of line intensities in the anti-Stokes and Stokes spectral regions. Applied research is aimed at using Raman spectroscopy and fluorescence microscopy in biomedical research, in particular, in the search for spectral markers of NETosis, as well as some features of lipid-protein interactions.
Expected results in the current year:
Investigation of a possible anomaly in the ratio of aSt/St peaks in SERS spectra depending on the pump power.
Elucidation of conformational changes in the Raman spectrum of proteins in the presence of membrane mimetics.
Light-induced NETosis upon excitation of neutrophil cells by radiation in the visible region of the spectrum: identification of mechanisms and signaling pathways.
Application of fluorescence microscopy to identify programmed death of neutrophil cells under the influence of various activators, including UV- and visible radiation.
List of projects |
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Project |
Leaders |
Priority |
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1. |
BIOPHOTONICS |
G.M.
Arzumanyan |
1 (2021-2023) |
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|
Activity or Experiment |
Leaders |
Status |
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Laboratory
or other |
Main researchers |
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1. |
Study
of the features of Stokes |
G.M. Arzumanyan |
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|
FLNP |
Ye. Arynbek, K.Z. Mamatkulov, I.A. Morkovnikov, 2 engineers |
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2. |
Determination
of the range of pump |
G.M.
Arzumanyan |
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|
FLNP |
Ye.
Arynbek, A.A. Shutikov, 1 engineer |
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3. |
Stabilization
of membrane proteins and |
G.M.
Arzumanyan |
|
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|
FLNP |
A. Damir, K.Z. Mamatkulov, A.A. Shutikov, D.S. Zakritnaya |
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4. |
Testing
the technique for obtaining Raman spectra of liposomes / lipodiscs
with |
K.Z.
Mamatkulov |
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|
FLNP |
Ye. Arynbek, A. Damir, A.A. Shutikov, D.S. Zakrinaya, 1 engineer |
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5. |
Study
of the influence of lipid environment |
G.M.
Arzumanyan |
|
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|
FLNP |
Ye. Arynbek, K.Z. Mamatkulov, 1 engineer, 2 laboratory assistants |
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6. |
Search
for spectral / Raman markers |
G.M.
Arzumanyan |
|
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|
FLNP |
Ye
Arynbek, E.M. Demina, I.M. Pugachevskaya, A.A. Shutikov, |
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7. |
Study
of mechanisms of sterile activation |
G.M.
Arzumanyan |
|
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FLNP |
Ye.
Arynbek, E.M. Demina, D.S. Kravtsunova, A.A. Shtikov, |
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8. |
Mastering
the technique of ultra-low |
G.M.
Arzumanyan |
|
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|
FLNP |
Ye. Arynbek, A.A. Shutikov, 2 engineers |
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Collaboration
Country or International Organization |
City |
Institute or Laboratory |
Armenia |
Yerevan |
Foundation ANSL |
Belarus |
Minsk |
BSUIR |
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|
SOL instruments |
India |
Aizawl |
MZU |
Kazakhstan |
Almaty |
INP |
Poland |
Krakow |
JU |
Romania |
Cluj-Napoca |
UBB |
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Magurele |
NIMP |
Russia |
Moscow |
GPI RAS |
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MSU |
Serbia |
Belgrade |
Univ. |
Slovakia |
Kosice |
UPJS |
Uzbekistan |
Jizzakh |
JSPI |
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